Summary: GSI Chandler Smolts

• Microsatellite DNA
• Differentiate Yakima Basin Chinook populations using microsatellite DNA
• Population of origin of smolts
• Identify population of origin of smolts passing Chandler
• Smolt production
• Use genetic data towards the quantification and comparison of smolt production in upper Yakima versus Naches Rivers
• 1500 smolt samples
• Analyses based on 1500 smolt samples drawn randomly from a temporally stratified collection

Questions

• Can we reduce sample size from 1500 to a smaller collection, without compromising results?
• Is 1500 sample appropriate, or should we be sampling more?

PowerPoint Presentation

The Model

• Repeat 100 times
• For each modeled sample size
• Separate analyses
• 2005 and 2006 data
• Therefore
• For each sample size 100 estimated number of smolts for Naches and upper Yakima – for both 2005 and 2006

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Questions

• Can we reduce sample size from 1500 to a smaller collection, without compromising results?
• Is 1500 sample appropriate, or should we be sampling more?

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The Model

• Repeat 100 times
• For each modeled sample size
• Separate analyses
• 2005 and 2006 data
• Therefore
• For each sample size 100 estimated number of smolts for Naches and upper Yakima – for both 2005 and 2006

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Conclusions

• Appropriate sample size depends on desired level of precision
• Variability: Temporally (season and year) and population
• Precision (and associated error) is a function of sampling NOT genetics methods
• Other sources of error: GSI, run expansion

Acknowledgements